LOCUS       pLV-[hsa-miR-648]        9148 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7069..7929
                     /label=Amp(R)
     promoter        8130..8670
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5674..5908
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6483..6938)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5980..6110
                     /label=SV40 pA
     misc_feature    6111..6324
                     /label=SV40 ori
     misc_feature    4995..5591
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3613
                     /label=intron
     CDS             3660..4985
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3403
                     /label=hsa-mir-648 precursor
BASE COUNT     2167 a      2375 c      2513 g      2093 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca ccagtctgtt 
     3121 aagcatttgc cagcacagta ctgcagtggg tgaagtaaat actgccctcc tgcagtccac 
     3181 agtgtctgga ggggaccaat agacagatga tcacagacac ctccaagtgt gcagggcact 
     3241 ggtgggggcc ggggcaggcc cagcgaaagt gcaggacctg gcacttagtc ggaagtgagg 
     3301 gtgcagttga gcaggaactg agggtgacgg gccagcacag ccgcatctgg gcgaggctga 
     3361 ggcagaggag cagacgggca gctggtttgc tgccgcggaa tgagctagct cgagcttttg 
     3421 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 
     3481 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 
     3541 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 
     3601 tcttccattt caggtgtcgt gaaaactacc cctctagagt cgagctaccg gtcgccacca 
     3661 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg 
     3721 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 
     3781 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct 
     3841 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc 
     3901 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg 
     3961 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg 
     4021 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 
     4081 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg 
     4141 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg 
     4201 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca 
     4261 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac 
     4321 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggac ccaccggtcg 
     4381 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 
     4441 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 
     4501 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
     4561 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
     4621 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
     4681 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
     4741 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
     4801 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
     4861 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
     4921 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 
     4981 cctgagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 
     5041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 
     5101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 
     5161 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 
     5221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 
     5281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 
     5341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 
     5401 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 
     5461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 
     5521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 
     5581 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta 
     5641 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 
     5701 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 
     5761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     5821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     5881 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     5941 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 
     6001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     6061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 
     6121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     6181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     6241 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta 
     6301 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa 
     6361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
     6421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 
     6481 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     6541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     6601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     6661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     6721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     6781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     6841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     6901 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 
     6961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     7021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     7081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     7141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     7201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     7261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     7321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     7381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     7441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     7501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     7561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     7621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     7681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     7741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     7801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     7861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     7921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     7981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     8041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     8101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     8161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     8221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 
     8281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     8341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     8401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     8461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     8521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     8581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     8641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     8701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 
     8761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 
     8821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 
     8881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     8941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     9001 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     9061 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct 
     9121 cactaaaggg aacaaaagct ggagctgc 
//
